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vap b proteintech  (Proteintech)


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    Proteintech vap b proteintech
    Vap B Proteintech, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 71 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vap+b+proteintech/VAPB+Antibody/pm40883511-234-170-171
    Average 95 stars, based on 71 article reviews
    vap b proteintech - by Bioz Stars, 2026-10
    95/100 stars

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    Recombinant:

    Article Title: Peri-mitochondrial actin filaments inhibit Parkin assembly by disrupting ER-mitochondria contacts.
    Article Snippet: .. Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models cell lines Human U2-OS ATCC HTB-96 Human HeLa ATCC CCL-2 Mouse MEF Losón et al, 2013 N/A Reagent/resource Reference or source Identifier or catalog number Recombinant DNA GFP-F-tractin Johnson and Schell, 2009 N/A Mito-BFP Korobova et al, 2013 N/A ER-tag-RFP E. Snapp, Albert Einstein College of Medicine, NY N/A Myc-VAP-B Gomez-Suaga et al, 2017 N/A pLAMP1-mCherry Amy Palmer Addgene: #45147 Cyto-CFP-FRBx5 Takanari Inoue Addgene: #103776 pEGFP-Parkin-WT Edward Fon Addgene: #45875 pcDNA-DEST47 PINK1 C-GFP Mark Cookson Addgene: #13316 pMXs-IP HA-Parkin Noboru Mizushima Addgene: # 38248 EGFP-LC3 Karla Kirkegaard Addgene: #11546 AKAP1-YFP-FKBP Gyorgy Haznoczky, Thomas Jefferson University, Philadelphia N/A ER-RFP-Mito Gyorgy Haznoczky, Thomas Jefferson University, Philadelphia N/A PM-RFP-Mito Gyorgy Haznoczky, Thomas Jefferson University, Philadelphia N/A mtGCampf6 Gyorgy Haznoczky, Thomas Jefferson University, Philadelphia N/A RFP-GFP-tf(Fis1) reporter system Christopher Shoemaker, Geisel School of Medicine, Dartmouth, NH N/A Antibodies Tom-20 Abcam Cat# ab78547 VAP-B Proteintech Cat# 66191-1-Ig PTPIP51 Proteintech Cat# 20541-1-AP anti-myc-tag Abcam Cat# ab32 INF2 Cell Signaling Technology Cat# 41081 VAP-B Proteintech Cat# 14477-1-AP LC3B Cell Signaling Technology Cat# 12741S Parkin Cell Signaling Technology Cat# 2132S 16 EMBO reports © The Author(s) D ow nloaded from https://w w w .em bopress.org on Septem ber 7, 2025 from IP 2405:4803:dadf:58d0:bc82:365e:71c7:1d84. .. Reagent/resource Reference or source Identifier or catalog number Parkin (phosphoS65) Abcam Cat# 315376 Pink1 Cell Signaling Technology Cat# 6946S Β-Actin Cell Signaling Technology Cat# 3700S MFN2 Cell Signaling Technology Cat# 9482S VDAC Invitrogen Cat# MA5-41088 Phosphor-Ubiquitin S65 Cell Signaling Technology Cat# 60802S HRP-conjugated anti-mouse IgG Bio-Rad Cat#1705047 HRP-conjugated anti-rabbit IgG Bio-Rad Cat#1706515 Alexa-Fluor coupled 488 anti-rabbit Invitrogen Cat# A11008 Alexa-Fluor coupled 647 anti-rabbit Invitrogen Cat# A21245 Oligonucleotides and other sequence-based reagents si-FMNL1 IDT hs.FMNL1.13.5 si-FMNL2 IDT hs.FMNL1.13.1 si-FMNL3 Ambion s-40551 si-Arp2 IDT (Custom synthesized) N/A si-MFN2 IDT hs.Mfn2.13.1 si-VDAC1 IDT hs.VDAC1.13.1 Negative Control IDT 51-01-14-04 RT-PCR primers This study Materials section Chemicals, enzymes and other reagents DMEM Corning Cat# 10013-CV DMEM Gibco Cat# 21063-029 Fetal Bovine Serum Sigma-Aldrich Cat# F4135 OPTI-MEM Gibco Cat# 31985062 Lipofectamine 2000 Invitrogen Cat# 11668 Lipofectamine RNAi max Invitrogen Cat# 13778 ECL Prime Western Blotting Detection Reagent GE healthcare Cat# 28980926 Polyvinylidene fluoride membrane EMD Millipore Cat# IPFL00010 Glutaraldehyde Electron Microscopy Sciences Cat# 16020 PFA Electron Microscopy Sciences Cat# 15710 Laemmli sample buffer Bio-Rad Cat#161-0747 ProLong Gold antifade mounting media Invitrogen Cat# P36930 DMSO Invitrogen Cat# D12345 CK666 Sigma-Aldrich Cat# SML0006 CCCP Sigma-Aldrich Cat# C2759 Reagent/resource Reference or source Identifier or catalog number FCCP MedChemExpress Cat# 100410 TMRE Sigma-Aldrich Cat# 87917 Antimycin A Sigma-Aldrich Cat# A8674 Oligomycin A Sigma-Aldrich Cat# 75351 Rotenone Sigma-Aldrich Cat# R8875 Rapamycin Sigma-Aldrich Cat# R0395 Histamine Sigma-Aldrich Cat# H7250 Ethidium Bromide Sigma-Aldrich Cat# E7637 Glucose Sigma-Aldrich Cat# 49163 Galactose Sigma-Aldrich Cat# 1287700 Duolink in situ Red Mouse/Rabbit starter kit Sigma-Aldrich Cat# DUO92101 PhalloidinAlexaFluor 488 Invitrogen Cat# A12379 MycoAlert Plus Mycoplasma detection kit ATCC Cat# 30-1012K Universal Mycoplasma detection kit Lonza Cat# LT07-701 Software Fiji Image Analysis ImageJ https://imagej.net/Fiji Prism version 10.1.0 GraphPad https:// www.graphpad.com/ scientificsoftware/ prism/ Office Excel Microsoft https:// www.microsoft.com/ en-gb/ Fusion Andor version 2.0.0.15 NIS-Elements Nikon Version 4.0 Zen Black Zeiss N/A Other Glass-bottom imaging dish MatTek Cat#P35G-1.5-14-C



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    RIDα regulated LD accumulation independently of Rab7 and RILP. (A and B) Representative confocal images from shNPC1-RIDα cells stained with antibodies to FLAG-RIDα (green) and HRS (red) (A) or late endosome/lysosome markers LAMP1 and LAMP2 (red) (B). (C) Postnuclear supernatants fractionated on 27% Percoll density gradients immunoblotted for RIDα and organelle-specific marker proteins (EGFR, plasma membrane; Rab5 and Rab7, early and late endosomes, respectively). (D) Equal protein aliquots immunoblotted with antibodies to Rab7 to monitor siRNA knockdown efficiency and to tubulin for protein loading control. (E) Representative confocal images from A549 cells without (top panels) or with (bottom panels) stable RIDα expression following treatment with NC or Rab7-specific siRNAs. Cells were stained with Bodipy 493/503 (green), DAPI (blue), and FLAG antibody (red). (F) RIDα immune complexes from stable cell lines expressing wild-type RIDα or RIDα (75-SS) resolved by SDS-PAGE for immunological detection with FLAG-epitope antibody. (G) Representative confocal images from stable cell lines expressing wild-type RIDα or RIDα (75-SS) stained with antibodies to β-COP (red) and FLAG-RIDα (green). (H) Representative confocal image of sterol-loaded shNPC1 cells expressing RILP-defective RIDα (75-SS) stained with FLAG-RIDα antibody (red), Bodipy 493-503 (green), and DAPI (blue). (A, G, and H) Boxed areas are magnified 2× to visualize individual and merged images to the right of each image. (A, B, E, G, and H) All size bars, 5 μm. (C and F) Representative immunoblots from two independent experiments.

    Journal: Journal of Virology

    Article Title: Adenovirus Modulates Toll-Like Receptor 4 Signaling by Reprogramming ORP1L-VAP Protein Contacts for Cholesterol Transport from Endosomes to the Endoplasmic Reticulum

    doi: 10.1128/JVI.01904-16

    Figure Lengend Snippet: RIDα regulated LD accumulation independently of Rab7 and RILP. (A and B) Representative confocal images from shNPC1-RIDα cells stained with antibodies to FLAG-RIDα (green) and HRS (red) (A) or late endosome/lysosome markers LAMP1 and LAMP2 (red) (B). (C) Postnuclear supernatants fractionated on 27% Percoll density gradients immunoblotted for RIDα and organelle-specific marker proteins (EGFR, plasma membrane; Rab5 and Rab7, early and late endosomes, respectively). (D) Equal protein aliquots immunoblotted with antibodies to Rab7 to monitor siRNA knockdown efficiency and to tubulin for protein loading control. (E) Representative confocal images from A549 cells without (top panels) or with (bottom panels) stable RIDα expression following treatment with NC or Rab7-specific siRNAs. Cells were stained with Bodipy 493/503 (green), DAPI (blue), and FLAG antibody (red). (F) RIDα immune complexes from stable cell lines expressing wild-type RIDα or RIDα (75-SS) resolved by SDS-PAGE for immunological detection with FLAG-epitope antibody. (G) Representative confocal images from stable cell lines expressing wild-type RIDα or RIDα (75-SS) stained with antibodies to β-COP (red) and FLAG-RIDα (green). (H) Representative confocal image of sterol-loaded shNPC1 cells expressing RILP-defective RIDα (75-SS) stained with FLAG-RIDα antibody (red), Bodipy 493-503 (green), and DAPI (blue). (A, G, and H) Boxed areas are magnified 2× to visualize individual and merged images to the right of each image. (A, B, E, G, and H) All size bars, 5 μm. (C and F) Representative immunoblots from two independent experiments.

    Article Snippet: Antibodies and reagents used in the study are described in and , respectively. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Target Source Vendor Catalog no. Actin Rabbit Sigma (St. Louis, MO) A2066 EGFR Rabbit Fitzgerald (Concord, MA) 20R-ER004 FLAG Mouse Sigma F3165 GFP Mouse Stressgen (San Diego, CA) SAB-500 HRS Rabbit Bethyl (Montgomery, TX) A300-989A Lamin B1 Rabbit Abcam (Cambridge, MA) ab16048 LAMP1 Mouse DSHB b H4A3 LAMP2 Mouse DSHB H4B NF-κB (p65) Rabbit Cell Signaling (Beverly, MA) 8242 NF-κB (pSer536) Rabbit Cell Signaling 3033 ORP1L Mouse Santa Cruz (San Diego, CA) sc-376602 ORP1L Rabbit Abcam ab203352 ORP5 Rabbit Novus (Littleton, CO) NBP2-19683 Rab5 Mouse BD Biosciences (San Jose, CA) 610281 Rab7 Rabbit Santa Cruz sc6563 SREBP1 Mouse Novus NB600-582 TLR4 Mouse R&D Systems (Minneapolis, MN) MAB14782 TfR Mouse Zymed (South San Francisco, CA) 13-6800 Tubulin Rabbit Cell Signaling 2125 VAP-A Rabbit Sigma HPA009174 VAP-B Mouse Proteintech (Rosemont, IL) 14477-1-AP Open in a separate window a Antibodies were routinely used at a dilution of 1:1,000 to 1:2,000 for immunoblotting and at a dilution of 1:100 to 1:500 for immunostaining. b DSHB, Developmental Studies Hybridoma Bank (developed under the auspices of the Eunice Kennedy Shriver National Institute of Child Health and Human Development [NICHD] and maintained by The University of Iowa Department of Biological Sciences [Iowa City, IA]).

    Techniques: Staining, Marker, Clinical Proteomics, Membrane, Knockdown, Control, Expressing, Stable Transfection, SDS Page, FLAG-tag, Western Blot

    Description of antibodies used in the study a

    Journal: Journal of Virology

    Article Title: Adenovirus Modulates Toll-Like Receptor 4 Signaling by Reprogramming ORP1L-VAP Protein Contacts for Cholesterol Transport from Endosomes to the Endoplasmic Reticulum

    doi: 10.1128/JVI.01904-16

    Figure Lengend Snippet: Description of antibodies used in the study a

    Article Snippet: Antibodies and reagents used in the study are described in and , respectively. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Target Source Vendor Catalog no. Actin Rabbit Sigma (St. Louis, MO) A2066 EGFR Rabbit Fitzgerald (Concord, MA) 20R-ER004 FLAG Mouse Sigma F3165 GFP Mouse Stressgen (San Diego, CA) SAB-500 HRS Rabbit Bethyl (Montgomery, TX) A300-989A Lamin B1 Rabbit Abcam (Cambridge, MA) ab16048 LAMP1 Mouse DSHB b H4A3 LAMP2 Mouse DSHB H4B NF-κB (p65) Rabbit Cell Signaling (Beverly, MA) 8242 NF-κB (pSer536) Rabbit Cell Signaling 3033 ORP1L Mouse Santa Cruz (San Diego, CA) sc-376602 ORP1L Rabbit Abcam ab203352 ORP5 Rabbit Novus (Littleton, CO) NBP2-19683 Rab5 Mouse BD Biosciences (San Jose, CA) 610281 Rab7 Rabbit Santa Cruz sc6563 SREBP1 Mouse Novus NB600-582 TLR4 Mouse R&D Systems (Minneapolis, MN) MAB14782 TfR Mouse Zymed (South San Francisco, CA) 13-6800 Tubulin Rabbit Cell Signaling 2125 VAP-A Rabbit Sigma HPA009174 VAP-B Mouse Proteintech (Rosemont, IL) 14477-1-AP Open in a separate window a Antibodies were routinely used at a dilution of 1:1,000 to 1:2,000 for immunoblotting and at a dilution of 1:100 to 1:500 for immunostaining. b DSHB, Developmental Studies Hybridoma Bank (developed under the auspices of the Eunice Kennedy Shriver National Institute of Child Health and Human Development [NICHD] and maintained by The University of Iowa Department of Biological Sciences [Iowa City, IA]).

    Techniques: